immunogenic effect of purified antigens of B Alexandrina against S Mansoni in experimental animals [II] as measured by the histopathological changes in the liver

The objective of this work was to test the immunogenic effect of 2 purified non-infected B. alexandrina hepatopancreas through histopathological changes in liver of Swiss albino mice [15 - 20 g]. Gel filtration chromatography was used to fractionate the crude antigen into 5 fractions, followed by re-fractionation and determination of their molecular weights by sodium dodecyl sulfate polyacrylamide gel electrophoresis [SDS-PAGE]. Four dilutions of Fiv [20000 - 29000 daltons] and Fv [20000 - 240000 daltons] were injected in 2 groups of mice [33 each] at weekly intervals and another control group was injected with phosphate buffer saline [PBS] in the same manner. Sacrification was done at 7 weeks from infection with 100 S. mansoni cercariae through immersion method. The results revealed that there were marked histopathological changes in liver of the control group in comparison with the 2 vaccinated groups, which appeared more or less normal with slight inflammatory infiltrate

Studies on dermanyssid and laelaptid mites as a foundation for assessing their role in causation and transmission of diseases under our geographical zone

The present study includes survey of mite species infesting three different rodents in the region of Al-Alamin, in the north western coast. Analysis of data revealed six different species of parasitic mites: Dermanyssus sp., Hirstionyssus sp., and Laelaps sp. 2 were isolated from G. andersoni, while Allodermanyssus sp. and Ornithonyssinus sp. were recorded from Mesocricetes auratus and Lealaps sp. 1 was caught from R. rattus. These species belong to two different families Dermanyssidae [4 species] and Laelaptidae [2 species] and they represent five different genera. All trials made to identify the six species refer that they are unknown ones and probably being as new species, but the final decision waits the reply of Dr. Macfarlane of the Common wealth Institute of Entomology, London. Since the species are considered as unknown, a modified key was constructed for identification of the families and the genera, and the unknown species were fully described and illustrated and were distinguished from the most related species. Description was done from mounted material which is kept in the Department of Parasitology, Faculty of Medicine, University of Al-Azhar and is on request for further investigations